The effect of a polyherbal formulation on the glycemic and algesic states in persistent hyperglycemic in non-diabetic mice

The present study was carried out to evaluate the effect of polyherbal formulation (DiarunPlus) on the blood glucose changes and nociceptive threshold and tolerance on non-diabetic mice (which rendered hyperglycemic with exogenous administration of dextrose). This study was carried in 2 phases, during phase-I, the mice were rendered hyperglycemic by repeated injections of dextrose (2g/kg i.p.) at 9,13 and 17 hours for a consecutive period of 3 days. In phase-II, the treatment period extended to 10 days similar to that of phase-I .The blood glucose level and pain threshold and pain tolerance were measured for the effect of diarun-plus on algesic state in persistent hyperglycemic mice. During phase-I (for 3 days), diarun-plus (0.5 or 1g/kg) produced mild reduction in blood glucose level, pain threshold (hind paw licking) and pain tolerance (jumping time) were found to be unaltered on fourth day. During phase II (for 10 days) blood glucose levels were lowered by diarun-plus(0.5 or 1g/ kg) on 11th day,but not statistically significant. Pain threshold and pain tolerance were significantly increased on 11th day. This present study establishes the protective effect of diarun-plus in preventing the complications of hyperglycemia without inducing adverse hypoglycemia , hence this polyherbal formulation can be used an adjuvant in the treatment of diabetes mellitus associated with neuronal damage causing peripheral pain especially